TRIM5/RNF88 (alpha isoform) Antibody

SKU: orb19133

Description

Goat polyclonal antibody to TRIM5

Images & Validation

• Tested Applications: ELISA, IHC
• Dilution Range: ELISA: 1:32000, WB: 0.3 μg/ml, IHC-P: 3-6µg/ml
• Predicted Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Kidney shows cytoplasm staining in PCT. Recommended concentration, 3-6ug/ml.WB: Preliminary experiments gave an approx 37kDa band in Lysates of Human cell lines Daudi, Jurkat and MOLT4 after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 56.3kDa according to NP_149023.1. The 37kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: TRIM5 / RNF88 (alpha isoform)
• Protein Sequence: CNIEKNENYQPK
• Molecular Weight: 56.3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti TRIM5 antibody, anti tripartite motif-containing 5 antibody, anti HGNC:16276 antibody, anti RNF88 antibody, anti tripartite motif protein 5 antibody, anti tripartite motif protein TRIM5 antibody, anti TRIM5alpha antibody, anti OTTHUMP00000069812 antibody, anti tripartite motif protein TRIM antibody, anti tripartite motif-containing protein 5 alpha antibody

orb19133 (4ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Quick Database Links

Gene Symbol

TRIM5 / RNF88 (alpha isoform)

NCBI Gene ID

85363