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Catalog Number | orb2276287 |
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Category | Antibodies |
Description | TRIM29 antibody validated for immunohistochemistry on 76 different Normal Tissues |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | Biorbyt-629M |
Tested applications | IHC |
Reactivity | Human |
Isotype | IgG2b, kappa |
Immunogen | Recombinant fragment (126 Amino acid residues between aa 1-200) of human TRIM29 protein |
Dilution range | 1:100-1:200 |
Conjugation | Unconjugated |
UniProt ID | Q14134 |
Storage | Antibody with azide – store at 2 to 8°C. Antibody without azide – store at -20 to -80°C. Antibody is stable for 24 months. Non- hazardous. No MSD required. |
Alternative names | Ataxia telangiectasia group D complementing gene ( Read more... |
Note | For research use only |
Application notes | Positive Control: Tonsil: A strong membranous and cytoplasmic TRIM29 immunostaining should be seen in squamous epithelial cells. Negative Control: Tonsil: All non-epithelial cells must not show any TRIM29 immunostaining. Cellular Localization: Cytoplasmic and cell surface Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions. |
Expiration Date | 12 months from date of receipt. |
Anal canal Squamous cell carcinoma with strong TRIM29 immunostaining of tumor cells.
Prostate A moderate intensity predominantly membranous TRIM29 staining is seen in basal cells of the prostate.
Tonsil surface epithelium A strong membranous and cytoplasmic TRIM29 immunostaining is seen in squamous epithelial cells of the tonsil surface.