TRIM29/ATDC Antibody

SKU: orb20473

Description

Goat polyclonal antibody to TRIM29

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, WB
• Dilution Range: ELISA: 1:128000, WB: 0.1-1 μg/ml, IHC-P: 3.75 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 70kDa band observed in Human Peripheral Blood Mononucleocytes lysates (calculated MW of 65.8kDa according to NP_036233.2). Recommended concentration: 0.3-1 μg/ml. An additional band of unknown identity was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: TRIM29 / ATDC
• Protein Sequence: EKDRIKSFTTNE
• Molecular Weight: 65.8; 37.3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti ataxia-telangiectasia group D-associated protein antibody, anti ATDC antibody, anti FLJ36085 antibody, anti tripartite motif protein TRIM29 antibody, anti tripartite motif-containing 29 antibody, anti TRIM29 antibody

Western blot analysis of Peripheral Blood Lymphocytes lysate using TRIM29 antibody

Immunohistochemical staining of Human Skin using TRIM29 antibody

Quick Database Links

Gene Symbol

TRIM29 / ATDC

NCBI Gene ID

23650