TRIM25 Antibody

• Catalog Number: orb1238684
• Category: Antibodies
• Description: TRIM25 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: TRIM25 antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human TRIM25.The immunogen is located within amino acids 520 - 570 of TRIM25.
• Concentration: 1 mg/mL
• Dilution range: TRIM25 antibody can be used for detection of TRIM25 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL. Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: TRIM25
• UniProt ID: Q14258
• NCBI: NP_005073
• Storage: TRIM25 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: TRIM25 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: TRIM25 Antibody: EFP, Z147, RNF147, ZNF147, EFP, E
• Note: For research use only
• Application notes: TRIM25 antibody can be used for detection of TRIM25 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL. Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of TRIM25 in HeLa cell lysate in (A) the absence and (B) presence of blocking peptide with TRIM25 antibody at 1 µg/mL.

Immunocytochemistry of TRIM25 in HeLa cells with TRIM25 antibody at 20 µg/mL.

Immunofluorescence of TRIM25 in HeLa cells with TRIM25 antibody at 20 µg/mL.