TRIM25 Antibody

SKU: orb1537859

Description

TRIM25 Antibody

Images & Validation

• Tested Applications: ELISA, ICC, IF, IHC, IHC-P, WB
• Dilution range: ELISA, ICC (20 µg/ml), IF, IHC, IHC-P (10 µg/ml), WB (1 - 2 µg/ml)
• Reactivity: Human, Mouse
• Application Notes:
  Further information: TRIM25 antibody can be used for detection of TRIM25 by Western blot at 1 ug/mL. Antibody can also be used for immunocytochemistry starting at 20 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot:

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: A 19 AA synthetic peptide near the C-Terminus of human TRIM25. The immunogen is located within aa 520-570 of TRIM25.
• Target: TRIM25
• Purification: Peptide Column Affinity Chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS containing 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

TRIM25, EFP, Zinc finger protein 147, Z147, Zinc finger protein-147, ZNF147, Tripartite motif containing 25, RING finger protein 147, RNF147, Tripartite motif-containing 25

Immunocytochemistry of TRIM25 in HeLa cells with TRIM25 antibody at 20 µg/ml.

Immunofluorescence of TRIM25 in HeLa cells with TRIM25 antibody at 20 ug/ml.

Western blot of TRIM25 in HeLa cell lysate in (A) the absence and (B) absence of blocking peptide with TRIM25 antibody at 1 ug/ml.

Quick Database Links

Gene Symbol

TRIM25