Tri-Methyl-Histone H3 (Lys27) Antibody

• Catalog Number: orb685608
• Category: Antibodies
• Description: This Tri-Methyl-Histone H3 (Lys27) Antibody is an unconjugated polyclonal product. It tagets HIST1H3A using a Peptide sequence around Tri-Methylation site of lysine 27(A-R-K(tri-methyl)-S-A) derived from Human Histone H3 as the immunogen. This antibody is suitable for ELISA, IF, IHC, WB.
• Target: HIST1H3A
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: liquid
• Buffer/Preservatives: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy u
• Immunogen: Peptide sequence around Tri-Methylation site of lysine 27(A-R-K(tri-methyl)-S-A) derived from Human Histone H3.
• UniProt ID: P68431
• Tested applications: ELISA, IF, IHC, WB
• Dilution range: WB: 1: 500-1: 1000, IHC-P: 1: 50-1: 100, IF: 1: 100-1: 200
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-H3/a antibody, anti- H3/c antibody, anti- H3/
• Research Area: Epigenetics & Chromatin
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of HT29 and C6 cells using Tri-Methyl-Histone H3 (Lys27) antibody

Immunohistochemical staining of human breast carcinoma tissue using Tri-Methyl-Histone H3 (Lys27) antibody

Immunofluorescence analysis of MEF cells using Tri-Methyl-Histone H3 (Lys27) antibody