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Histone H3 Rabbit Polyclonal Antibody (Nuclear Loading Control)
Description
Research Area
Images & Validation
−| Tested Applications | FC, ICC, IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution Range | WB=1:5000-50000, IHC-P=1:500-2000, IHC-F=1:500-2000, ICC/IF=1:100, IF=1:500-2000, Flow-Cyt=1μg/Test |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Hamster, Insect, Yeast |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human Histone H3 (71-136/136aa) |
| Target | H3C1 |
| Molecular Weight | 15 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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UniProt Details
−Protocol Information
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Ruta, Veronica et al. Inhibition of Polycomb Repressive Complex 2 activity reduces trimethylation of H3K27 and affects development in Arabidopsis seedlings BMC Plant Biol., 19, 429 (2019)
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A Lepri et al. Architecture of light-independent seed germination: a multi-layered regulation in the model plant Cardamine hirsuta iris.uniroma1.it, (2026)
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Zhu, Jianning et al. Inhibiting inflammation and modulating oxidative stress in oxalate-induced nephrolithiasis with the Nrf2 activator dimethyl fumarate Free Radic Biol Med, 134, (2019)
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