TRC8 Antibody

• Catalog Number: orb1246462
• Category: Antibodies
• Description: TRC8 Antibody
• Target: RNF139
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Canine
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-HTGAAAEEFNDDT
• UniProt ID: Q8WU17
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 75kDa band observed in Human Skeletal Muscle lysates (calculated MW of 76.0kDa according to NP_009149.2). Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:In paraffin embedded Human Placenta shows membranous staining in the cytotrophoblasts. Recommended concentration, 3-4ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: RNF139, ring finger protein 139, TRC8, HRCA1, MGC3
• Research Area: Cancer Biology, Cell Biology
• Note: For research use only
• NCBI: NP_009149.2

orb1246462 (1 ug/ml) staining of Human Skeletal Muscle lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1246462 (3.8 ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.