Goat anti-TRC8 Antibody

• Catalog Number: orb20189
• Category: Antibodies
• Description: Goat polyclonal antibody to RNF139
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Canine, Human
• Dilution range: ELISA: 1:16000, WB: 0.3-1 μg/ml, IHC-P: 3-4µg/ml
• Conjugation: Unconjugated
• MW: 76
• Target: TRC8
• Entrez: 11236
• Protein Sequence: HTGAAAEEFNDDT
• RRID: AB_10755892
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti RNF139 antibody, anti ring finger protein 139
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.IHC: In paraffin embedded Human Placenta shows membranous staining in the cytotrophoblasts. Recommended concentration, 3-4μg/ml.WB: Experiments gave bands at approx. 150kDa and 50kDa in Human Skeletal Mucle lysate after 0.5 μg/ml antibody staining. This protein has a calculated MW of 76.0kDa according to NP_009149.2.The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.5-1.5 μg/ml.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of formalin-fixed and paraffin embedded human Placenta tissue using TRC8 antibody

Western blot analysis of Human Skeletal Muscle lysate using RNF139 antibody