TRC8 Antibody

SKU: orb20189

Description

Goat polyclonal antibody to RNF139

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:16000, WB: 0.3-1 μg/ml, IHC-P: 3-4µg/ml
• Reactivity: Human
• Predicted Reactivity: Canine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.IHC: In paraffin embedded Human Placenta shows membranous staining in the cytotrophoblasts. Recommended concentration, 3-4μg/ml.WB: Experiments gave bands at approx. 150kDa and 50kDa in Human Skeletal Mucle lysate after 0.5 μg/ml antibody staining. This protein has a calculated MW of 76.0kDa according to NP_009149.2.The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.5-1.5 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: TRC8
• Molecular Weight: 76
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti RNF139 antibody, anti ring finger protein 139 antibody, anti TRC8 antibody, anti HRCA1 antibody, anti MGC31961 antibody, anti RCA1 antibody, anti patched related protein translocated in renal cancer antibody, anti multiple membrane spanning receptor TRC8 antibody

Immunohistochemical analysis of formalin-fixed and paraffin embedded human Placenta tissue using TRC8 antibody

Western blot analysis of Human Skeletal Muscle lysate using RNF139 antibody

Quick Database Links

Gene Symbol

TRC8

NCBI Gene ID

11236