Trastuzumab Antibody

SKU: orb1297802

Description

Trastuzumab is a high-affinity humanized monoclonal antibody targeting HER2, used in research on HER2-positive cancers. It exhibits antitumor activity by inducing cell cycle arrest, mediating cytotoxicity, and inhibiting DNA repair, making it applicable for both in vitro and in vivo experimental models.

Research Area

Cardiovascular Research, Signal Transduction

Key Properties

• CAS Number: 180288-69-1
• MW: 145.36 kDa
• Purity: 99.50%
• Target: ADC Antibody,EGFR

Bioactivity

• Target IC50:
  MCF-7 cells viability:1660 µg/mL (72h)|JIMT-1 cells viability:9.7 μM|AMJ13 cells viability:1780 µg/mL (72h)
• In Vivo:
  METHODS: To test the in vivo antitumor activity, Trastuzumab (5 µg/g) was administered intraperitoneally once a week for seven doses to nude mice harboring human mammary carcinoma tumor JIMT-1. RESULTS: Trastuzumab had a significant inhibitory effect on tumor growth on days 21-56. After stopping administration, tumors began to grow in two nude mice whose tumors were palpable earlier.
• In Vitro:
  METHODS: Human breast cancer cells BT-474 were treated with Trastuzumab (0.01-10 µg/mL) for 72 h. Cell viability was determined by MTS Assay. RESULTS: The antiproliferative effect of Trastuzumab was observed at concentrations as low as 0.5 µg/mL, with a stabilizing level of approximately 10 µg/mL. METHODS: CHO-K6, CHO-EGFR and CHO-HER3 cells were treated with Trastuzumab (0.1-10 µg/mL) for 5-60 min, and binding to HER receptors was detected by Immunofluorescence. RESULTS: Trastuzumab binds only to HER2, but not to EGFR and HER3.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ADC Antibody, ADCAntibody, Anti-Human HER2, Humanized Antibody, inhibit, Inhibitor, HER1, ErbB-1, Epidermal growth factor receptor, EGFR, Trastuzumab

Quick Database Links

Gene Symbol

ADC Antibody,EGFR

UniProt

P04626