Anti-erbB-2 (Her-2/neu) [4D5-8 (trastuzumab)]

SKU: orb357997

Description

Recombinant monoclonal antibody to erbB-2 (Her-2/neu)

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, FC, IHC
• Reactivity: Human

Key Properties

• Host: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 4D5-8 (trastuzumab)
• Immunogen: A431 cells (human epidermoid carcinoma) (over)expressing EGFR.
• Target: erbB-2 (Her-2/neu)
• Purity: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

CD340; Metastatic lymph node gene 19 protein (MLN 19); Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2; p185erbB2; p185HER2; EGFR

Immunohistochemical staining of paraffin embedded human breast tissue using anti-erbB-2 antibody 4D5-8 (trastuzumab) Anti-erbB-2 (Her-2/neu) staining of paraffin embedded human breast tissue using the rabbit-chimeric version of 4D5-8 (trastuzumab) (orb348902). Antigen retreival was achieved by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with orb348902 was carried out at 4 µg/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3, 3′-diaminobenzidine), and counter-staining with haemotoxylin. Weak membrane staining exclusively of ductal epithelial cells may be observed. Recommended concentration, 4-6 µg/ml.

Quick Database Links

Gene Symbol

erbB-2 (Her-2/neu)

UniProt

P04626