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Description
Research Area
Stem Cell & Developmental Biology
Images & Validation
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| Application Notes |
|---|
Key Properties
−| Source | Human Cells |
|---|---|
| Target | Tnfsf11 |
| Tag | C-6 His tag |
| MW | 28.5 kD |
| Purity | Greater than 95% as determined by reducing SDS-PAGE.Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test. |
| Protein Sequence | Ala73-Asp316 |
Storage & Handling
−| Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Lyophilized from a 0.2 um filtered solution of 20mM PB, 150mM NaCl, pH 7.4. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Tumor necrosis factor ligand superfamily member 11, Tnfsf11, Osteoclast differentiation factor, ODF, Osteoprotegerin ligand, OPGL, Receptor activator of nuclear factor kappa-B ligand, RANKL, TNF-related activation-induced cytokine, TRANCE, CD254, RANK L
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The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 48.4 kDa after removal of the signal peptide.The apparent molecular mass of hFc-TNFSF11 is approximately 55-70 kDa due to glycosylation.
Mammalian
100 μg, 10 μg, 50 μg Recombinant Mouse RANK Protein [orb2994208]
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SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
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10 μg, 50 μg, 500 μg, 1 mgRecombinant Human RANK Protein [orb2994416]
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SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 21.1 KDa. Observed: 25-30 KDa, reducing conditions
50 μg, 500 μg, 1 mg, 10 μgRecombinant Mouse RANK L Protein [orb2994453]
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SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE. (QC verified)
Predicted: 28.3 KDa. Observed: 30-35 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mg- Item 1 of 3
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Protein Handling and Storage Guide
Protein Handling Guide
TRANCE Recombinant Protein (orb1228470)
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