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| Catalog Number | orb2994453 |
|---|---|
| Category | Proteins |
| Description | Recombinant Mouse TNF-related Activation-induced Cytokine is produced by our Mammalian expression system and the target gene encoding Arg43-Asp287 is expressed with a 6His tag at the N-terminus. |
| Conjugation | Unconjugated |
| Tag | N-6His |
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl,8% Trehalose,2% Mannitol,0.05% Tween80,pH 9.0. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Purity | SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE. (QC verified) |
| UniProt ID | BAA97257.1 |
| MW | Predicted: 28.3 KDa. Observed: 30-35 KDa, reducing conditions |
| Expression System | Human Cells |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Biological Origin | Mouse |
| Biological Activity | BLI: Loaded Recombinant Human OPG-Fc(Cat#orb3002306) on Pro A Biosensor, can bind Mouse RANKL-His(Cat#orb2994453) with an affinity constant of 1.02 pM as determined in BLI assay. |
| Expression Region | Arg43-Asp287 |
| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| Alternative names | Tumor necrosis factor ligand superfamily member 11 Read more... |
| Note | For research use only |
| Expiration Date | 6 months from date of receipt. |
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 90% as determined by SEC-HPLC.(QC verified) | |
Predicted: 23 KDa. Observed: 23-29 KDa, reducing conditions. |
> 95% by SDS-PAGE. | |
Recombinant Mouse TNFSF11/RANK L/TRANCE Protein is produced by E. coli expression system. The target protein is expressed with sequence (Gln137-Asp316) of mouse TNFSF11/RANK L/TRANCE (Accession #O35235). |
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