Human TNFSF11 Protein, hFc Tag

• Catalog Number: orb757436
• Category: Proteins
• Description: Recombinant human TNFSF11 Protein with N-terminal Human Fc tag
• Target: TNFSF11
• Tag: N-Human Fc Tag
• Reactivity: Human
• Form/Appearance: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Buffer/Preservatives: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
• Purity: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
• Purification: Purified from cell culture supernatant by affinity chromatography
• UniProt ID: O14788
• MW: The protein has a predicted molecular mass of 48.4 kDa after removal of the signal peptide.The apparent molecular mass of hFc-TNFSF11 is approximately 55-70 kDa due to glycosylation.
• Source: Mammalian
• Storage: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
• Alternative names: CD254;hRANKL2;ODF;OPGL;OPTB2;RANKL;sOdf;TNLG6B;TRA
• Research Area: Developmental Biology, Epigenetics
• Note: For research use only
• Expiration Date: 6 months from date of receipt.

Human CD27 Protein, hFc Tag on SDS-PAGE under reducing condition.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human CD27, hFc tagged protein (orb689459) can bind Anti-CD27 Neutralizing antibody orb689323 in a linear range of 3.2-80.0 ng/ml.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human CD27, hFc tagged protein (orb689459) can bind Human CD70, mFc-His tagged protein orb689401 in a linear range of 3.2-400 ng/ml.

Flow cytometry analysis with 15 μg/mL Human CD27 Protein, hFc tag (orb689459) on Expi293 cells transfected with human CD70 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).