Human TNFSF11 Protein, hFc Tag

SKU: orb757436

Description

Recombinant human TNFSF11 Protein with N-terminal Human Fc tag

Key Properties

• Source: Mammalian
• Target: TNFSF11
• Reactivity: Human
• Tag: N-Human Fc Tag
• Molecular Weight: The protein has a predicted molecular mass of 48.4 kDa after removal of the signal peptide.The apparent molecular mass of hFc-TNFSF11 is approximately 55-70 kDa due to glycosylation.
• Purification: Purified from cell culture supernatant by affinity chromatography
• Purity: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.

Storage & Handling

• Storage: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
• Form/Appearance: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Buffer/Preservatives: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
• Disclaimer: For research use only

Alternative Names

CD254;hRANKL2;ODF;OPGL;OPTB2;RANKL;sOdf;TNLG6B;TRANCE

Human CD27 Protein, hFc Tag on SDS-PAGE under reducing condition.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human CD27, hFc tagged protein (orb689459) can bind Anti-CD27 Neutralizing antibody orb689323 in a linear range of 3.2-80.0 ng/ml.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human CD27, hFc tagged protein (orb689459) can bind Human CD70, mFc-His tagged protein orb689401 in a linear range of 3.2-400 ng/ml.

Flow cytometry analysis with 15 μg/mL Human CD27 Protein, hFc tag (orb689459) on Expi293 cells transfected with human CD70 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

Quick Database Links

Gene Symbol

TNFSF11

UniProt

O14788