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TPO Antibody

SKU: orb1248832

Description

TPO Antibody

Images & Validation

Tested ApplicationsELISA, IHC, WB
ReactivityHuman
Application Notes
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 110kDa band observed in Human Thyroid Gland lysates (calculated MW of 103kDa according to NP_000538.3). Recommended concentration: 0.3-1µg/mL.Immunohistochemistry:In paraffin embedded Human Thyroid Gland shows textured staining in the cytoplasm of epithelial cells. Recommended concentration, 5-10µg/mL.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: C-TRHVIQVSNEVVTDD
TargetTPO
Molecular WeightApprox 110 kDa
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

TPO, thyroid peroxidase, MSA, TDH2A, TPX, OTTHUMP00000115535, OTTHUMP00000115536, thyroid microsomal antigen, thyroperoxidase

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UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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0.1 mg
$ 920.00
Free Secondary Antibody (20 ul)0/0

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DispatchUsually dispatched within 5-10 working days
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