TPO Antibody

• Catalog Number: orb1248832
• Category: Antibodies
• Description: TPO Antibody
• Target: TPO
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-TRHVIQVSNEVVTDD
• UniProt ID: P07202
• MW: Approx 110 kDa
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 110kDa band observed in Human Thyroid Gland lysates (calculated MW of 103kDa according to NP_000538.3). Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:In paraffin embedded Human Thyroid Gland shows textured staining in the cytoplasm of epithelial cells. Recommended concentration, 5-10ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: TPO, thyroid peroxidase, MSA, TDH2A, TPX, OTTHUMP0
• Note: For research use only
• NCBI: NP_783653.1, NP_000538.3, NP_783650.1, NP_783652.1

orb1248832 (0.3 ug/ml) staining of Human Thyroid Gland lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248832 (5 ug/ml) staining of paraffin embedded Human Thyroid Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.