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Human Anti-Thyroid-Peroxidase Antibody (TPO-Ab) ELISA Kit

Catalog Number: orb782852

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb782852
CategoryAssays and Kits
DescriptionThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with anti-Thyroid-Peroxidase antibody(TPO-Ab) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to anti-Thyroid-Peroxidase antibody(TPO-Ab).
ReactivityHuman
Concentration200 ng/mL
Tested applicationsELISA
Application notesstandard: 200 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TPO-Ab. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TPO-Ab. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TPO-Ab, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TPO-Ab in the samples is then determined by comparing the OD of the samples to the standard curve
Assay Time3.5h
Range3.13-200 ng/mL
Sensitivity1.14 ng/mL
Sample Typesserum, plasma, cell lysates, cell culture supernates and other biological fluids
Alternative namesTPO Ab ELISA Kit, TPO-Ab ELISA Kit
NoteFor research use only
Human Anti-Thyroid-Peroxidase Antibody (TPO-Ab) ELISA Kit