Human Anti-Thyroid-Peroxidase Antibody (TPO-Ab) ELISA Kit

• Catalog Number: orb782852
• Category: Assays and Kits
• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with anti-Thyroid-Peroxidase antibody(TPO-Ab) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to anti-Thyroid-Peroxidase antibody(TPO-Ab).
• Reactivity: Human
• Concentration: 200 ng/mL
• Tested applications: ELISA
• Application notes: standard: 200 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TPO-Ab. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TPO-Ab. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TPO-Ab, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TPO-Ab in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 3.13-200 ng/mL
• Sensitivity: 1.14 ng/mL
• Sample Types: serum, plasma, cell lysates, cell culture supernates and other biological fluids
• Alternative names: TPO Ab ELISA Kit, TPO-Ab ELISA Kit
• Research Area: Immunology & Inflammation, Infectious Disease & Vi
• Note: For research use only