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Human Anti-Thyroid-Peroxidase Antibody (TPO-Ab) ELISA Kit

Catalog Number: orb782852

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb782852
CategoryAssays and Kits
DescriptionThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with anti-Thyroid-Peroxidase antibody(TPO-Ab) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to anti-Thyroid-Peroxidase antibody(TPO-Ab).
Tested applicationsELISA
ReactivityHuman
Range3.13-200 ng/mL
Assay Time3.5h
Concentration200 ng/mL
Sensitivity1.14 ng/mL
Alternative namesTPO Ab ELISA Kit, TPO-Ab ELISA Kit
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NoteFor research use only
Application notesstandard: 200 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TPO-Ab. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TPO-Ab. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TPO-Ab, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TPO-Ab in the samples is then determined by comparing the OD of the samples to the standard curve
Sample Typesserum, plasma, cell lysates, cell culture supernates and other biological fluids
Expiration DatePlease enquire.
Human Anti-Thyroid-Peroxidase Antibody (TPO-Ab) ELISA Kit