Recombinant Topoisomerase II alpha Antibody / TOP2A

SKU: orb2637102

Description

Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and re-ligating the broken strand. [UniProt]

Images & Validation

• Tested Applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 0.5-1ug/ml
• Reactivity: Human, Mouse
• Application Notes:
  Optimal dilution of the recombinant Topoisomerase II alpha antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Recombinant
• Isotype: Mouse IgG1, kappa
• Clone No.: rTOP2A/6629
• Immunogen: A portion of amino acids 1352-1493 was used as the immunogen for the recombinant Topoisomerase II alpha antibody.
• Purification: Protein A/G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

SDS-PAGE analysis of purified, BSA-free recombinant Topoisomerase II alpha antibody (clone rTOP2A/6629) as confirmation of integrity and purity.

IHC staining of FFPE human lymph node with recombinant Topoisomerase II alpha antibody (clone rTOP2A/6629). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human tonsil tissue with recombinant Topoisomerase II alpha antibody (clone rTOP2A/6629). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Quick Database Links

UniProt

P11388