Topoisomerase II alpha Antibody / TOP2A

• Catalog Number: orb1151532
• Category: Antibodies
• Description: Rabbit monoclonal antibody to Topoisomerase II alpha
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: TOP2A/6570R
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: A portion of amino acids 1352-1493 was used as the immunogen for the recombinant Topoisomerase II alpha antibody.
• Antibody Type: Primary Antibody
• Dilution range: Western blot: 2-4ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant Topoisomerase II alpha antibody is available for research use only.
• UniProt ID: P11388
• Storage: Aliquot the recombinant Topoisomerase II alpha antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant Topoisomerase II alpha antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human lymph node tissue with recombinant Topoisomerase II alpha antibody (clone TOP2A/6570R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human tonsil tissue with recombinant Topoisomerase II alpha antibody (clone TOP2A/6570R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Negative control: IHC staining of FFPE human brain tissue using recombinant Topoisomerase II alpha antibody (clone TOP2A/6570R) at 2 ug/ml in PBS for 30 min RT. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human HeLa and Jurkat cell lysates using recombinant Topoisomerase II alpha antibody (clone TOP2A/6570R). Predicted molecular weight ~174 kDa.

SDS-PAGE analysis of purified, BSA-free recombinant Topoisomerase II alpha antibody (clone TOP2A/6570R) as confirmation of integrity and purity.