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Topoisomerase II alpha Antibody / TOP2A

Catalog Number: orb1151532

DispatchUsually dispatched within 5-10 working days
$ 780.00
Catalog Numberorb1151532
CategoryAntibodies
DescriptionRabbit monoclonal antibody to Topoisomerase II alpha
ClonalityRecombinant
Species/HostRabbit
IsotypeRabbit IgG
ConjugationUnconjugated
ReactivityHuman
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
PurityProtein A/G affinity
ImmunogenA portion of amino acids 1352-1493 was used as the immunogen for the recombinant Topoisomerase II alpha antibody.
UniProt IDP11388
Tested applicationsIHC-P, WB
Dilution rangeWestern blot: 2-4ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application notesOptimal dilution of the recombinant Topoisomerase II alpha antibody should be determined by the researcher.
Antibody TypePrimary Antibody
Clone NumberTOP2A/6570R
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
StorageAliquot the recombinant Topoisomerase II alpha antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Hazard InformationThis recombinant Topoisomerase II alpha antibody is available for research use only.
NoteFor research use only
Topoisomerase II alpha Antibody / TOP2A

IHC staining of FFPE human lymph node tissue with recombinant Topoisomerase II alpha antibody (clone TOP2A/6570R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Topoisomerase II alpha Antibody / TOP2A

IHC staining of FFPE human tonsil tissue with recombinant Topoisomerase II alpha antibody (clone TOP2A/6570R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Topoisomerase II alpha Antibody / TOP2A

Negative control: IHC staining of FFPE human brain tissue using recombinant Topoisomerase II alpha antibody (clone TOP2A/6570R) at 2 ug/ml in PBS for 30 min RT. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Topoisomerase II alpha Antibody / TOP2A

Western blot testing of human HeLa and Jurkat cell lysates using recombinant Topoisomerase II alpha antibody (clone TOP2A/6570R). Predicted molecular weight ~174 kDa.

Topoisomerase II alpha Antibody / TOP2A

SDS-PAGE analysis of purified, BSA-free recombinant Topoisomerase II alpha antibody (clone TOP2A/6570R) as confirmation of integrity and purity.

  • Topoisomerase II alpha Antibody / TOP2A [orb750075]

    IF,  IHC-P,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    20 μg
  • Topoisomerase II alpha Antibody / TOP2A [orb750076]

    IF,  IHC-P,  WB

    Human, Mouse

    Mouse

    Monoclonal

    Unconjugated

    20 μg
  • Topoisomerase II alpha Antibody / TOP2A [orb2640298]

    IF,  IHC-P,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    100 μg
  • Topoisomerase II alpha Antibody / TOP2A [orb2640300]

    IF,  IHC-P,  WB

    Human, Mouse

    Mouse

    Monoclonal

    Unconjugated

    100 μg
  • Topoisomerase II alpha Antibody / TOP2A [orb1151540]

    IHC-P,  WB

    Human, Mouse

    Mouse

    Recombinant

    Unconjugated

    100 μg