Topoisomerase I Rabbit mAb

• Catalog Number: orb1564156
• Category: Antibodies
• Description: Topoisomerase I Rabbit mAb
• Clonality: Monoclonal
• Clone Number: R02-2H9
• Tested applications: ICC, IF, IHC-Fr, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic peptide of human Topoisomerase I
• Antibody Type: Primary Antibody
• Concentration: 0.3 mg/ml
• Dilution range: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Calculated MW: 91 kDa; Observed MW: 91 kDa
• Target: TOP1
• UniProt ID: P11387
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% rAlbumin.
• Alternative names: TOP1; DNA topoisomerase 1; DNA topoisomerase I
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Topoisomerase I in K562, rat Brain, C6, 3T3, Hela lysates using Topoisomerase I antibody.

Immunocytochemistry analysis of Topoisomerase I (green) in Hela using Topoisomerase I antibody, and DAPI (blue).

Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Topoisomerase I antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.