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Topoisomerase I Rabbit Polyclonal Antibody

Catalog Number: orb499925

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb499925
CategoryAntibodies
DescriptionTopoisomerase I Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IHC-Fr, IHC-P, WB
Predicted ReactivityBovine, Equine, Gallus, Porcine, Rabbit, Sheep
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human Topoisomerase I (401-500/765aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Form/AppearanceLiquid
ConjugationUnconjugated
MW91 kDa
TargetTOP1
UniProt IDP11387
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesDNA topoisomerase 1; DNA topoisomerase I; NUP98 fu
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NoteFor research use only
Expiration Date12 months from date of receipt.
Topoisomerase I Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Topoisomerase I) Polyclonal Antibody, Unconjugated (orb499925) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Topoisomerase I Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Topoisomerase I) Polyclonal Antibody, Unconjugated (orb499925) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Topoisomerase I Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Topoisomerase I) Polyclonal Antibody, Unconjugated (orb499925) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Topoisomerase I Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Topoisomerase I) Polyclonal Antibody, Unconjugated (orb499925) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Topoisomerase I Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Topoisomerase I) Polyclonal Antibody, Unconjugated (orb499925) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Topoisomerase I Rabbit Polyclonal Antibody

Sample: Lane 1: Human LO2 cell lysates, Lane 2: Human MCF-7 cell lysates, Lane 3: Human SW480 cell lysates, Primary: Anti-Topoisomerase I (orb499925) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 91 kDa, Observed band size: 100 kDa.

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