TOP1MT Antibody

SKU: orb1252580

Description

TOP1MT Antibody

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Application Notes:
  Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the TOP1MT antibody should be determined by the researcher.1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b, kappa
• Clone No.: TOP1MT/488
• Immunogen: Recombinant full-length human protein was used as the immunogen for the TOP1MT antibody.
• Target: TOP1MT
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Disclaimer: For research use only

Formalin-fixed, paraffin-embedded human gallbladder stained with TOP1MT antibody (TOP1MT/488).

Formalin-fixed, paraffin-embedded human melanoma stained with TOP1MT antibody (TOP1MT/488).

Formalin-fixed, paraffin-embedded human pancreas stained with TOP1MT antibody (TOP1MT/488).

SDS-PAGE Analysis of Purified, BSA-Free TOP1MT Antibody (clone TOP1MT/488). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

Gene Symbol

TOP1MT

UniProt

Q969P6