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Catalog Number | orb1252580 |
---|---|
Category | Antibodies |
Description | TOP1MT Antibody [TOP1MT/488] |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | TOP1MT/488 |
Tested applications | FC, IF, IHC-P, WB |
Reactivity | Human |
Isotype | IgG2b, kappa |
Immunogen | Recombinant full-length human protein was used as the immunogen for the TOP1MT antibody. |
Antibody Type | Primary Antibody |
Concentration | 0.2 mg/mL |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | TOP1MT |
UniProt ID | Q969P6 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
Note | For research use only |
Application notes | Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the TOP1MT antibody should be determined by the researcher.1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
Formalin-fixed, paraffin-embedded human gallbladder stained with TOP1MT antibody (TOP1MT/488).
Formalin-fixed, paraffin-embedded human melanoma stained with TOP1MT antibody (TOP1MT/488).
Formalin-fixed, paraffin-embedded human pancreas stained with TOP1MT antibody (TOP1MT/488).
SDS-PAGE Analysis of Purified, BSA-Free TOP1MT Antibody (clone TOP1MT/488). Confirmation of Integrity and Purity of the Antibody.
FC, IF, IHC | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |