Recombinant Topoisomerase I, Mitochondrial (TOP1MT) Antibody

• Catalog Number: orb1410322
• Category: Antibodies
• Description: Mouse monoclonal antibody to Topoisomerase I, Mitochondrial (TOP1MT)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: rTOP1MT/488
• Tested applications: FC, IF, IHC, WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: Recombinant full-length human TOP1MT protein
• Antibody Type: Recombinant Antibody
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Dilution range: Flow Cytometry (0.5-1ug/million cells); ,Immunofluorescence (0.5-1ug/ml); Western Blot (0.5-2ug/ml);,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Form/Appearance: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Conjugation: Unconjugated
• MW: 70kDa
• Target: TOP1MT
• Entrez: 116447
• UniProt ID: Q969P6
• Expression System: HEK293 cells
• Storage: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
• Buffer/Preservatives: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Alternative names: DNA topoisomerase I; TOP1MT; Topoisomerase I mitoc
• Note: For research use only
• Application notes: Positive Control: MCF-7 or A431 cells. Jurkat cell lysate. Human heart, skeletal muscle, brain or fetal liver tissue.
• Expiration Date: 12 months from date of receipt.