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TOM20 Antibody
Description
Images & Validation
−| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant protein of human TOM20 |
| Target | TOMM20 |
| Molecular Weight | Observed: 16kDa |
| Purification | Affinity purification |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Human Mitochondrial Import Receptor Subunit TOM20 Homolog (TOMM20) ELISA Kit [orb1950105]
Human
0.16-10 ng/mL
0.052 ng/mL
48 T, 96 TTOMM20 Antibody [orb632046]
ELISA, IF, IHC, WB
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
100 μg, 50 μgTOM20 Antibody [orb1474487]
IF, IHC, WB
Hamster, Human
Rabbit
Monoclonal
Unconjugated
30 μl, 100 μl, 200 μl, 50 μl

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Western blot analysis of extracts of various cell lines, using TOM20 antibody (orb1258835) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5min.

Immunohistochemistry of paraffin-embedded human colon carcinoma using TOM20 antibody (orb1258835) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human colon using TOM20 antibody (orb1258835) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human lung cancer using TOM20 antibody (orb1258835) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using TOM20 antibody (orb1258835) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat heart using TOM20 antibody (orb1258835) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of C6 cells using TOM20 antibody (orb1258835) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 using TOM20 antibody (orb1258835) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U2OS cells using TOM20 antibody (orb1258835) at dilution of 1:100. Blue: DAPI for nuclear staining.
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TOM20 Antibody (orb1258835)
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