TOM20 Antibody

• Catalog Number: orb1258835
• Category: Antibodies
• Description: TOM20 Antibody
• Target: TOMM20
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Immunogen: Recombinant protein of human TOM20
• UniProt ID: Q15388
• MW: Observed: 16kDa
• Tested applications: IF, IHC, WB
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: MAS2MOM19, TOM2mitochondrial import receptor subun
• Note: For research use only

Western blot analysis of extracts of various cell lines, using TOM20 antibody (orb1258835) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5min.

Immunohistochemistry of paraffin-embedded human colon carcinoma using TOM20 antibody (orb1258835) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human colon using TOM20 antibody (orb1258835) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human lung cancer using TOM20 antibody (orb1258835) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using TOM20 antibody (orb1258835) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat heart using TOM20 antibody (orb1258835) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of C6 cells using TOM20 antibody (orb1258835) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 using TOM20 antibody (orb1258835) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U2OS cells using TOM20 antibody (orb1258835) at dilution of 1:100. Blue: DAPI for nuclear staining.