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| Catalog Number | orb1256783 |
|---|---|
| Category | Antibodies |
| Description | TOM20 Antibody |
| Target | TOMM20 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Form/Appearance | Liquid |
| Concentration | batch dependent |
| Buffer/Preservatives | Liquid |
| Purification | Affinity purification |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 20-145 of human TOM20 (NP_055580.1). |
| UniProt ID | Q15388 |
| MW | Observed: 16kDa |
| Tested applications | IF, IHC, WB |
| Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:20 - 1:50 |
| Antibody Type | Primary Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | MAS2MOM19, TOM2mitochondrial import receptor subun Read more... |
| Research Area | Cancer Biology, Signal Transduction |
| Note | For research use only |

Immunohistochemistry of paraffin-embedded mouse heart using TOM20 antibody (orb1256783) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using TOM20 antibody (orb1256783) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Immunohistochemistry of paraffin-embedded human colon using TOM20 antibody (orb1256783) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human colon carcinoma using TOM20 antibody (orb1256783) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human liver cancer using TOM20 antibody (orb1256783) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat ovary using TOM20 antibody (orb1256783) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat heart using TOM20 antibody (orb1256783) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of A549 cells using TOM20 antibody (orb1256783). Blue: DAPI for nuclear staining.

Immunohistochemistry of paraffin-embedded human liver cancer using MTA3 antibody (orb1256865) at dilution of 1:100 (40x lens).
Human | |
0.16-10 ng/mL | |
0.052 ng/mL |
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Monoclonal | |
Unconjugated |
ELISA, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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