TNNT3 Antibody

• Catalog Number: orb1248444
• Category: Antibodies
• Description: TNNT3 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: The immunogen for this antibody is: C-TTLRSRIDQAQKHSK
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 35kDa band observed in Human, Mouse and Rat Skeletal Muscle lysates (calculated MW of 30.6kDa according to NP_006748.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Marian et al, Circ Res. 1997 Jul;81(1):76-85. PMID: 9201030.).Recommended concentration: 0.01-0.03ug/ml.Immunohistochemistry:In paraffin embedded Human Skeletal Muscle shows staining of select fibrils. Recommended concentration, 4-8ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Approx 35kDa
• Target: TNNT3
• UniProt ID: P45378
• NCBI: NP_001036246.1, NP_001036247.1, NP_006748.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: TNNT3, troponin T type 3 (skeletal, fast), TNTF, b
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 35kDa band observed in Human, Mouse and Rat Skeletal Muscle lysates (calculated MW of 30.6kDa according to NP_006748.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Marian et al, Circ Res. 1997 Jul;81(1):76-85. PMID: 9201030.).Recommended concentration: 0.01-0.03ug/ml.Immunohistochemistry:In paraffin embedded Human Skeletal Muscle shows staining of select fibrils. Recommended concentration, 4-8ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248444 (0.01 ug/ml) staining of Human (A), Mouse (B) and Rat (C) Skeletal Muscle lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248444 (5 ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.