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Description
Research Area
Immunology & Inflammation
Images & Validation
−
Key Properties
−| Source | E. coli |
|---|---|
| Biological Activity | The ED50 was determined by the cytolysis of murine L929 cells in the presence of actinomycin D is 5 x 107 units/mg. |
| Target | Tnf |
| Purity | Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg). |
| Protein Sequence | MLRSSSQNSS DKPVVHVVAN HQAEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLIY SQVLFKGQGC PDYVLLTHTV SRFATSYQEK VSLLSAIKSP CPKDTPEGAE LKPWYEPMYL GGVSQLEKGD LLSAEVNLPK YLDITESGQV YFGVIAL |
Storage & Handling
−| Storage | The lyophilized TNF alpha recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted TNF alpha is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing TNF alpha recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature. |
|---|---|
| Form/Appearance | Lyophilized |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Tnfa, RATTNF, TNF-alpha, Tnfa, Tnfsf2, Tumor necrosis factor, Cachectin, TNF-a
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Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_036807.1 |
|---|
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Datasheet
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
TNF alpha Recombinant Protein (orb1230646)
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