Human TNFa Protein, His Tag

• Catalog Number: orb689423
• Category: Proteins
• Description: Recombinant human TNFa protein with C-terminal 6×His tag
• Reactivity: Human
• Tag: C-6×His Tag
• Form/Appearance: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Purity: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
• MW: The protein has a predicted molecular mass of 18.1 kDa after removal of the signal peptide.
• Target: TNFA
• UniProt ID: P01375
• Source: Mammalian
• Storage: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
• Buffer/Preservatives: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
• Alternative names: DIF;TNF-alpha;TNFA;TNFSF2;cachexin;cachectin;TNFα
• Note: For research use only
• Expiration Date: 6 months from date of receipt.

Human TNFα Protein, His Tag on SDS-PAGE under reducing condition.

ELISA plate pre-coated by 1 μg/ml (100 μL/well) Human TNFa, His tagged protein (orb689423) can bind Anti-TNFa Neutralizing antibody ([getskuurl sku"orb1173644"]) in a linear range of 0.64-16 ng/ml.

ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human TNFα Protein, His Tag (orb689423) can bind Anti-TNFa (Certolizumab biosimilar) mAb (orb1826906) in a linear range of 0.64–16 ng/ml.

ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human TNFα Protein, His Tag (orb689423) can bind Anti-TNFa (golimumab biosimilar) mAb (orb1826910) in a linear range of 3.20–80 ng/ml.