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Catalog Number | orb689423 |
---|---|
Category | Proteins |
Description | Recombinant human TNFa protein with C-terminal 6×His tag |
Reactivity | Human |
Tag | C-6×His Tag |
Form/Appearance | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
Purity | The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
Conjugation | Unconjugated |
MW | The protein has a predicted molecular mass of 18.1 kDa after removal of the signal peptide. |
Target | TNFα |
UniProt ID | P01375 |
Source | Mammalian |
Storage | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Buffer/Preservatives | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. |
Alternative names | DIF;TNF-alpha;TNFA;TNFSF2;cachexin;cachectin;TNFα Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
95% | |
19.1 kDa | |
Mouse TNF-alpha, His Tag (orb1496307) is expressed from human 293 cells (HEK293). It contains AA Leu 80 - Leu 235 (Accession # P06804). |
FA, HPLC, SDS-PAGE | |
Unconjugated | |
> 95.0% as determined by RP-HPLC and analysis by SDS-PAGE |
≥90% as determined by SDS-PAGE | |
This protein contains the human TNF(Val77-Leu233) was fused with the N-terminal His Tag and expressed in Mammalian cells. |
≥90% as determined by SDS-PAGE | |
This protein contains the human TNF(Val77-Leu233) was fused with the C-terminal His Tag and expressed in E. coli. |
≥90% as determined by SDS-PAGE | |
This protein contains the human TNF(Gly57-Leu233) was fused with the N-terminal His Tag and expressed in E. coli. |
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