Recombinant Human Tumor necrosis factor (TNF) Protein

SKU: orb624131

Description

This Recombinant Human Tumor necrosis factor (TNF) Protein spans the amino acid sequence from region 77-233aa. Purity: Greater than 90% as determined by SDS-PAGE.

Images & Validation

• Application Notes:
  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Key Properties

• Source: Yeast
• Biological Origin: Homo sapiens (Human)
• Biological Activity: Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 33.32-47.38 pg/mL
• Tag: N-terminal 6xHis-tagged
• Molecular Weight: 19.4 kDa
• Expression Region: 77-233aa
• Protein Length: Partial
• Protein Sequence: VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL
• Purity: Greater than 90% as determined by SDS-PAGE.

Storage & Handling

• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
• Form/Appearance: Liquid or Lyophilized powder
• Buffer/Preservatives: Lyophilized from 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0; Tris-based buffer,50% glycerol
• Disclaimer: For research use only

Alternative Names

Cachectin TNF-alpha Tumor necrosis factor ligand superfamily member 2

Measured by its binding ability in a functional ELISA. Immobilized Human TNF at 5 μg/ml can bind Human TNFR2 protein. The EC50 is 3.470-4.107 ng/mL.

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Quick Database Links

UniProt

P01375