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Recombinant Human Tumor necrosis factor (TNF) Protein
Catalog Number: orb624131
| Catalog Number | orb624131 |
|---|---|
| Category | Proteins |
| Description | Recombinant Human Tumor necrosis factor protein |
| Tag | N-terminal 6xHis-tagged |
| Form/Appearance | Liquid or Lyophilized powder |
| Buffer/Preservatives | Lyophilized from 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0; Tris-based buffer,50% glycerol |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Protein Sequence | VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL |
| Protein Length | Partial |
| UniProt ID | P01375 |
| MW | 19.4 kDa |
| Application notes | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
| Endotoxins | Not test. |
| Source | Yeast |
| Biological Origin | Homo sapiens (Human) |
| Biological Activity | Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 33.32-47.38 pg/mL |
| Expression Region | 77-233aa |
| Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
| Alternative names | Cachectin TNF-alpha Tumor necrosis factor ligand s Read more... |
| Research Area | Immunology & Inflammation |
| Note | For research use only |
| Expiration Date | 6 months from date of receipt. |
Measured by its binding ability in a functional ELISA. Immobilized Human TNF at 5 μg/ml can bind Human TNFR2 protein. The EC50 is 3.470-4.107 ng/mL.
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
- Item 1 of 1
Human TNF protein [orb705324]
Greater than 85% as determined by SDS-PAGE.
19.4 kDa
E.coli
20 μg, 100 μg, 1 mg - Item 1 of 1
Human TNFA protein (Active) [orb359192]
> 97% as determined by SDS-PAGE and HPLC.
18.3 kDa
E.Coli
10 μg, 100 μg, 500 μg - Item 1 of 1
Human TNFA protein (Active) [orb359193]
> 98% as determined by SDS-PAGE and HPLC.
16.9 kDa
E.Coli
10 μg, 100 μg, 500 μg - Item 1 of 1
Human TNFA protein (Active) [orb359194]
> 98% as determined by SDS-PAGE and HPLC.
17.5 kDa
E.Coli
10 μg, 100 μg, 500 μg - Item 1 of 1
Human CD40L protein (Active) [orb359197]
> 95% as determined by SDS-PAGE and HPLC.
16.3 kDa
E.Coli
10 μg, 100 μg, 500 μg
