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Catalog Number | orb624131 |
---|---|
Category | Proteins |
Description | Recombinant Human Tumor necrosis factor protein |
Tag | N-terminal 6xHis-tagged |
Form/Appearance | Lyophilized powder |
Purity | Greater than 90% as determined by SDS-PAGE. |
MW | 19.4 kDa |
UniProt ID | P01375 |
Protein Sequence | VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL |
Protein Length | Partial |
Source | Yeast |
Expression System | 77-233aa |
Biological Activity | Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 33.32-47.38 pg/mL. |
Expression Region | 77-233aa |
Endotoxins | Not test. |
Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
Buffer/Preservatives | Lyophilized from 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0 |
Alternative names | Cachectin TNF-alpha Tumor necrosis factor ligand s Read more... |
Note | For research use only |
Application notes | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
Expiration Date | 6 months from date of receipt. |
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Greater than 93% as determined by SDS-PAGE. | |
46.6 kDa | |
Mammalian cell |
Greater than 85% as determined by SDS-PAGE. | |
19.4 kDa | |
E.coli |
> 97% as determined by SDS-PAGE and HPLC. | |
18.3 kDa | |
E.Coli |
> 98% as determined by SDS-PAGE and HPLC. | |
16.9 kDa | |
E.Coli |
> 98% as determined by SDS-PAGE and HPLC. | |
17.5 kDa | |
E.Coli |
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