TNF alpha Recombinant Protein

SKU: orb1229855

Description

TNF alpha Recombinant Protein

Research Area

Cancer Biology, Cell Biology, Immunology & Inflammation, Metabolism Research, Signal Transduction

Images & Validation

• Application Notes:
  This recombinant protein can be used for biological assays. For research use only.

Key Properties

• Source: E. coli
• Biological Activity: ED50 is less than 0.03 ng/ml as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D.Specific Activity of 3.0 x 107 IU/mg.
• Target: TNF
• Tag: Tag Free
• Molecular Weight: 17.3 kD
• Protein Sequence: Val77-Leu233
• Purity: Greater than 95% as determined by reducing SDS-PAGE.Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.

Storage & Handling

• Storage: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Lyophilized from a 0.2 um filtered solution of 20mM PB, 150mM NaCl, pH 7.0. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Tumor Necrosis Factor, Cachectin, TNF-Alpha, Tumor Necrosis Factor Ligand Superfamily Member 2, TNF-a, TNF, TNFA, TNFSF2

Quick Database Links

Gene Symbol

TNF

UniProt

P01375

RefSeq:P01375