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Catalog Number | orb1229855 |
---|---|
Category | Proteins |
Description | TNF alpha Recombinant Protein |
Tag | Tag Free |
Form/Appearance | Lyophilized |
Purity | Greater than 95% as determined by reducing SDS-PAGE.Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test. |
MW | 17.3 kD |
Target | TNF |
UniProt ID | P01375 |
Protein Sequence | Val77-Leu233 |
Source | E. coli |
Biological Activity | ED50 is less than 0.03 ng/ml as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D.Specific Activity of 3.0 x 107 IU/mg. |
NCBI | P01375 |
Storage | Lyophilized protein should be stored at -20˚°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7˚°C for 2-7 days.Aliquots of reconstituted samples are stable at -20˚°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 um filtered solution of 20mM PB, 150mM NaCl, pH 7.0. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. |
Alternative names | Tumor Necrosis Factor, Cachectin, TNF-Alpha, Tumor Read more... |
Note | For research use only |
Application notes | This recombinant protein can be used for biological assays. For research use only. |
Expiration Date | 6 months from date of receipt. |
FC, ICC, IF, IHC-Fr, IHC-P, WB | |
Rat | |
Human, Mouse | |
Rabbit | |
Recombinant | |
Unconjugated |
Greater than 90% as determined by SDS-PAGE. | |
19.4 kDa | |
Yeast |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 18.1 kDa after removal of the signal peptide. | |
Mammalian |
Greater than 95% as determined by SDS-PAGE. | |
17.5 kDa | |
E.coli |
Greater than 95% as determined by SDS-PAGE. | |
18.5 kDa | |
E.coli |