You have no items in your shopping cart.
Featured
Description
Research Area
Immunology & Inflammation
Images & Validation
−
Item 1 of 1
| Tested Applications | ELISA, WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Human TNF-alpha, His Tag at 0.2 ug/mL (100 uL/well) can bind Human TNFR1, Fc Tag with a linear range of 0.4-6.25 ng/mL. |
| Target | TNF-alpha |
| Tag | His Tag |
| MW | 18.2 kDa |
| Purity | >95% as determined by SDS-PAGE.Endotoxin level is less than 1.0 EU per ug by the LAL method. |
| Protein Sequence | Val 77 - Leu 233 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | PBS, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−DIF,TNF-alpha,TNFA,TNFSF2,cachexin,cachectin,TNFalpha
Similar Products
−- Item 1 of 6
- Item 1 of 2
Recombinant Human Tumor necrosis factor (TNF) Protein [orb624131]
Greater than 90% as determined by SDS-PAGE.
19.4 kDa
Yeast
1 mg, 100 μg, 20 μg - Item 1 of 4
- Item 1 of 4
Human TNFa Protein, His Tag [orb689423]
Unconjugated
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 18.1 kDa after removal of the signal peptide.
Mammalian
100 μg, 10 μg, 50 μg - Item 1 of 3
Recombinant Human CD156b/ADAM17 Protein, N-His [orb2968206]
ELISA, SDS-PAGE, WB
>90% as determined by SDS-PAGE.
30.34 kDa
100 μg, 1 mg, 50 μg
Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].
Quick Database Links
Gene Symbol
TNF-alpha
RefSeq (Protein):NP_000585.2
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_000585.2 |
|---|
Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Available Sizes
Select a size below
Usually dispatched within 5-10 working days