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Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution Range | WB: 1:500-1:5000; IHC: 1:200-1:1000 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Immunogen | tumor necrosis factor(TNF superfamily, member 2) |
| Target | TNF alpha |
| Purity | ≥95% as determined by SDS-PAGE |
| Purification | Protein A+G purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | liquid |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNF-a, TNFSF2, Tumor necrosis factor
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LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with orb632005 (TNF-a Antibody) at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using orb632005 (TNF-a Antibody) at dilution of 1:800. heat mediated antigen retrieved with Tris-EDTA buffer (pH9).
Quick Database Links
Gene Symbol
TNF alpha
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
Request a Document
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
TNF alpha Mouse Monoclonal Antibody (orb632005)
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