Anti-TNF alpha FITC

• Catalog Number: orb179929
• Category: Antibodies
• Description: Mouse monoclonal antibody against TNF alpha conjugated to FITC.
• Target: TNF alpha
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Conjugation: FITC
• Reactivity: Human, Porcine, Primate
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purity: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Immunogen: Recombinant human TNF-alpha
• UniProt ID: P01375
• Tested applications: FC
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. Intracellular staining.
• Antibody Type: Primary Antibody
• Clone Number: MAb11
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Alternative names: anti APC1 antibody (FITC), anti Cachectin antibody
• Note: For research use only
• Entrez: 7124

Flow cytometry multicolor intracellular staining pattern of human PHA stimulated peripheral blood mononuclear cells stained using anti-human TNF alpha (MAb11) FITC antibody (4 μl reagent per milion cells in 100 μl of cell suspension). For surface staining was used anti-human CD3 (UCHT1) APC antibody (10 μl reagent per milion cells in 100 μl of cell suspension).

Separation of human TNF alpha positive CD3 positive cells (red-filled) from TNF alpha negative CD3 negative cells (black-dashed) in flow cytometry analysis (intracellular staining) of human PHA stimulated peripheral blood mononuclear cells stained using anti-human TNF alpha (MAb11) FITC antibody (4 μl reagent per milion cells in 100 μl of cell suspension).

Flow cytometry intracellular staining pattern of human PHA stimulated peripheral blood mononuclear cells stained using anti-human TNF alpha (MAb11) FITC antibody (4 μl reagent per milion cells in 100 μl of cell suspension).