TNF alpha antibody

• Catalog Number: orb420299
• Category: Antibodies
• Description: TNF alpha antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: This antibody was produced from whole rabbit serum prepared by repeated immunizations with recombinant mouse TNFα produced in E.coli.
• Concentration: 1.0 mg/ml
• Dilution range: ELISA: 1:1,000 - 1:5,000, WB: 1:500 - 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: TNF alpha Antibody has been heated to 56°C for 30 minutes. The antibody is directed against mature 17,000 MW mouse TNFα and is useful in determining its presence in various assays. The antibody does not recognize mouse TNFß (lymphotoxin).
• Conjugation: Unconjugated
• UniProt ID: P06804
• NCBI: P06804.2
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-TNF alpha antibody, rabbit anti-TNFa a
• Note: For research use only
• Application notes: This antibody against Mouse TNFα has been tested for use in immunoblotting. This antibody is suitable for ELISA, immunohistochemistry, neutralizations, radioimmunoassay, and immunoprecipitation. It recognizes the 17,000 MW TNFα. Reactivity in other immunoassays is unknown. This antibody will recognize the cell-bound precursor of TNFα as a 26,000 protein in immunoblots, particularly in denatured samples. This antibody is also useful for neutralization of mouse activity in bioassays. It does not neutralize the biological activity of lymphotoxin. For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.
• Expiration Date: 12 months from date of receipt.