TNF alpha Antibody

• Catalog Number: orb334996
• Category: Antibodies
• Description: Goat polyclonal antibody to TNF alpha. TNF-α is produced by a variety of immune cells including NK cells, B cells, T cells and macrophages. It is multifunctional proinflammatory cytokine that belongs to the tumour necrosis factor (TNF) superfamily. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is involved in the regulation of several processes including lipid metabolism cell differentiation and proliferation, coagulation and apoptosis. It has been implicated in a variety of diseases, including cancer and autoimmune diseases.
• Target: Tumor necrosis factor
• Clonality: Polyclonal
• Species/Host: Goat
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Bovine, Canine, Donkey, Equine, Feline, Gallus, Goat, Guinea pig, Hamster, Human, Mouse, Other, Porcine, Primate, Rabbit, Rat, Sheep
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS, 20% glycerol and 0.05% sodium azide
• Purification: Epitope affinity purified
• Immunogen: Antigen: Purified recombinant human TNF-_ produced in E. coli.. Antigen Sequence: MSTESMIRDVELAEEALPKKTGGPQGSRRCLFLSLFSFLIVAGATTLFCLLHFGVIGPQREEFPRDLSLISPLAQAVRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL
• Tested applications: WB
• Dilution range: WB:1:500-1:2,000
• Application notes: The antibody solution should be gently mixed before use.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: APC1, cachectin; tumor necrosis factor ligand supe
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of staining of total protein lysate using TNF alpha antibody