ANO1 Antibody

SKU: orb1252163

Description

ANO1 Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Application Notes:
  Flow Cytometry: 0.5-1 ug/million cells in 0.1mlIF: 0.5-1 µg/mLIHC (FFPE): 0.5-1 µg/mL for 30 minutes at RTThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the DOG1 antibody to be titered up or down for optimal performance.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b, kappa
• Clone No.: DG1/1486
• Immunogen: A recombinant human protein corresponding to amino acids 2-101 of human DOG1 was used as the immunogen for this DOG1 antibody.
• Target: ANO1
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TMEM16A Antibody: DOG1, TAOS2, ORAOV2, TMEM16A, DOG1, Anoctamin-1, Discovered on gastrointestinal stromal tumors protein 1

IHC testing of FFPE human gastrointestinal stromal tumor (GIST) with DOG1 antibody (DG1/1486). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.

SDS-PAGE Analysis of Purified, BSA-Free DOG1 Antibody (clone DG1/1486). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

Gene Symbol

ANO1

UniProt

Q5XXA6