TLR3 Antibody

• Catalog Number: orb1538005
• Category: Antibodies
• Description: TLR3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: A peptide corresponding to 15 AA near the C-Terminus of human TLR3. The immunogen is located within aa 780-830 of TLR3.
• Concentration: 1 mg/ml
• Dilution range: ELISA, ICC, IF, IHC, IHC-P (5 µg/ml), WB (1 - 2 µg/ml)
• Conjugation: Unconjugated
• Target: TLR3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS containing 0.02% Sodium Azide. PBS containing 0.02% Sodium Azide
• Alternative names: TLR3, CD283, Toll-like receptor 3, CD283 antigen,
• Note: For research use only
• Application notes: Further information: TLR3 antibody can be used for detection of TLR3 by Western blot at 0.5 to 2 ug/mL. Antibody can also be used for immunocytochemistry starting at 1 ug/mL and immunohistochemistry starting at 2 ug/mL. For immunofluorescence start at 10 ug/mL. Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human and mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested. Western Blot: Predicted: 99 kDa Observed: 105 kDa
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry of TLR3 in EL4 cells with TLR3 antibody at 1 µg/ml.

Immunofluorescence of TLR3 in El4 cells with TLR3 antibody at 10 ug/ml.

Western blot of TLR3 in Daudi cell lysate with TLR3 antibody at (A) 1 and (B) 2 ug/ml.