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TIP47 Antibody

Catalog Number: orb1240193

DispatchUsually dispatched within 5-10 working days
$ 670.00
Catalog Numberorb1240193
CategoryAntibodies
DescriptionTIP47 Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsELISA, IF, IHC-P, WB
Predicted ReactivityPorcine
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenAnti-TIP47 antibody (orb1240193) was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human TIP47. The immunogen is located within the last 50 amino acids of TIP47.
Antibody TypePrimary Antibody
Concentration1 mg/mL
Form/AppearanceLiquid
ConjugationUnconjugated
MWPredicted: 47kD Observed: 47kD
TargetPLIN3
UniProt IDO60664
NCBIO60664
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesTIP47 Antibody is supplied in PBS containing 0.02% sodium azide.
Alternative namesTIP47 Antibody: PP17, TIP47, M6PRBP1, Perilipin-3,
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NoteFor research use only
Expiration Date12 months from date of receipt.
TIP47 Antibody

Western Blot Validation in Human Daudi Cells. Loading: 15 µg of lysates per lane. Antibodies: TIP47 orb1240193, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 µg/mL, Lane2: 2 µg/mL.

TIP47 Antibody

Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: TIP47 orb1240193 (4 µg/mL), TIP47 orb1240194 (1 µg/mL), and beta-actin (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

TIP47 Antibody

Western Blot Validation in Rat Liver. Loading: 15 µg of lysates per lane. Antibodies: TIP47 orb1240193, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.5 µg/mL, Lane2: 1 µg/mL, Lane3: 2 µg/mL.

TIP47 Antibody

Immunohistochemistry Validation of TIP47 in Rat Liver Tissue. Immunohistochemical analysis of paraffin-embedded Rat Liver Tissue using anti-TIP47 antibody (orb1240193) at 10 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

TIP47 Antibody

Immunofluorescence Validation of TIP47 in Rat Liver Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed rat liver cells labeling TIP47 with orb1240193 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

TIP47 Antibody

Induced Expression Validation of TIP47 in Atg7 Liver KO Mice (Singh et al., 2009). TIP47 expression detected by anti-TIP47 antibodies was markedly increased in the liver of Atg7 liver KO mice.

TIP47 Antibody

Induced Expression Validation of TIP47 in Mouse Liver (Singh et al., 2009). TIP47 expression detected by anti-TIP47 antibodies was increased in the autophagic vacuoles (AVs) and lysosomes (Lys) after starvation.

TIP47 Antibody

Induced Expression Validation of TIP47 in HT29 Cells (Qi et al., 2013). TIP47 expression detected by anti-TIP47 antibodies was increased in the colonic cells after oleic acid treatment.

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    ELISA,  IHC,  IHC-P,  WB

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    ELISA,  IHC,  IHC-P,  WB

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