TIMD4 Antibody

SKU: orb1240179

Description

TIMD4 Antibody

Research Area

Immunology & Inflammation, Signal Transduction

Images & Validation

• Tested Applications: ELISA, ICC, IF, IHC-P, WB
• Reactivity: Human, Mouse
• Application Notes:
  TIM-4 antibody can be used for the detection of TIM-4 by Western blot at 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL and Immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human and mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: TIM-4 antibody was raised against a 16 amino acid synthetic peptide from near the center of human TIM-4.The immunogen is located within amino acids 60 - 110 of TIM-4.
• Target: TIMD4
• Molecular Weight: Observed: 45 kDa
• Purification: TIM-4 Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: TIM-4 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TIM-4 Antibody: TIM4, SMUCKLER, TIM4, T-cell immunoglobulin and mucin domain-containing protein 4, T-cell immunoglobulin mucin receptor 4, TIMD-4

Western blot analysis of TIM-4 in RAW264.7 cell lysate with TIM-4 antibody at 2 µg/mL in (A) the absence and (B) the presence of blocking peptide.

Immunocytochemistry of TIM-4 in Jurkat cells with TIM-4 antibody at 10 µg/mL.

Immunofluorescence of TIM-4 in Jurkat cells with TIM-4 antibody at 20 µg/mL.

Immunohistochemistry of TIM-4 in human spleen tissue with TIM-4 antibody at 2.5 µg/ml.

Immunofluorescence of TIM-4 in human spleen tissue with TIM-4 antibody at 20 µg/ml. Green: TIM-4 Antibody (orb1240179) Blue: DAPI staining.

Immunohistochemistry of TIM-4 in mouse liver tissue with TIM-4 antibody at 2 µg/ml.

Quick Database Links

Gene Symbol

TIMD4

UniProt

Q96H15

RefSeq (Protein):NP_612388