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Anti-Tim-4 [5G3]

SKU: orb613969

Description

Rat monoclonal antibody to Tim-4

Research Area

Cancer Biology, Immunology & Inflammation, Signal Transduction

Images & Validation

Tested ApplicationsELISA, FACS, FC, IP, In vivo
ReactivityMouse

Key Properties

HostRat
ClonalityMonoclonal
IsotypeIgG1
Clone No.5G3
ImmunogenThis antibody was generated by immunizing a female Lewis rat with Tim-4-Ig fusion protein.
TargetTim-4
PurityPurified
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPBS with 0.02% Proclin 300.
Concentration1 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

TIMD4; SMUCKLER; SMUCKLER, TIM4; Spleen, mucin-containing, knockout of lymphotoxin protein; T-cell immunoglobulin and mucin domain containing 4; T-cell immunoglobulin and mucin domain-containing protein 4; T-cell immunoglobulin mucin receptor 4; T-cell membrane protein 4; TIM-4; TIMD-4

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  • Anti-Tim-4 [5G3] [orb613968]

    ELISA,  FACS,  FC,  IP,  In vivo

    Mouse

    Rat

    Monoclonal

    Unconjugated

    0.2 mg
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Anti-Tim-4 [5G3]

Immunofluorescence staining of fixed mouse splenocytes with anti-Tim-4 antibody 5G3. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of 5G3 (orb613968) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb613968, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-unknown specificity antibody followed by Alexa Fluor® 488 secondary antibody.

UniProt Details

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Protocol Information

FACS
Fluorescence-Activated Cell Sorting (FC, Flow cytometry)
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FC
Flow Cytometry
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ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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IP
Immunoprecipitation
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Anti-Tim-4 [5G3] (orb613969)

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Available Sizes

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0.2 mg
$ 600.00
DispatchUsually dispatched within 5-10 working days
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