Thyroid Hormone Receptor Antibody (FITC)

SKU: orb612669

Description

Mouse monoclonal antibody against NR1D1 conjugated to FITC

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: WB (1:500); IHC (1:100)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  A 1:500 dilution of SMC-561 was sufficient for detection of Pan-Thyroid hormone receptor in 10 ug of Hep G2 Human Hepatoblastoma Cell lysate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: H43
• Immunogen: Synthetic peptide from the full length Human Thyroid hormone receptor protein
• Target: Thyroid Hormone Receptor
• Molecular Weight: 70 kDa
• Purification: Protein A Purified

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 640.91mM DMSO, 136.36 mM Ethanolamine, 126.89 mM chlorides, 9.09mM phosphates, 9.09mM NaHCO3
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

ERBA ALPHA, ERBA beta, ERBA1, ERBA2, NR1A1, NR1A2, THR1, THRA, THRA1, THRA2, THRB, THRB1, THRB2, Thyroid hormone receptor alpha, Thyroid hormone receptor beta

Immunohistochemistry analysis using Mouse Anti-Thyroid Hormone Receptor Monoclonal Antibody, Clone H43. Tissue: Thyroid. Species: Mouse. Primary Antibody: Mouse Anti-Thyroid Hormone Receptor Monoclonal Antibody at 1:100 for Overnight at 4°C, then 30 min at 37°C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37°C. Counterstain: DAPI for 3 min at RT. Magnification: 7.5X.

Immunohistochemistry analysis using Mouse Anti-Thyroid Hormone Receptor Monoclonal Antibody, Clone H43. Tissue: Thyroid Cancer. Species: Human. Primary Antibody: Mouse Anti-Thyroid Hormone Receptor Monoclonal Antibody at 1:100 for Overnight at 4°C, then 30 min at 37°C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37°C. Counterstain: DAPI for 3 min at RT. Magnification: 4X.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Thyroid Hormone Receptor Monoclonal Antibody, Clone H43. Tissue: Differentiated SH-SY5Y. Species: Human. Primary Antibody: Mouse Anti-Thyroid Hormone Receptor Monoclonal Antibody at 1:250. Secondary Antibody: AlexaFluor 488. Counterstain: phalloidin (Alexa 647, red), beta tubulin (Anti-beta III Tubulin Ab, Alexa 555, magenta) Hoechst (blue). (A) Phalloidin (B) Anti-beta III Tubulin Ab. (C) Thyroid Hormone Receptor Antibody. (D) Hoechst (E) Composite.

Western Blot analysis of Human Hep G2 Hepatoblastoma Cell lysate showing detection of Thyroid Hormone Receptor protein using Mouse Anti-Thyroid Hormone Receptor Monoclonal Antibody, Clone H43. Load: 10 ug. Primary Antibody: Mouse Anti-Thyroid Hormone Receptor Monoclonal Antibody at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Other Band (s): Higher molceular weight bands could be due to PTMs.

Quick Database Links

Gene Symbol

Thyroid Hormone Receptor

UniProt

P10827

NCBI Gene ID

7067

RefSeq (mRNA):NM_001190918.2