NCOA3 Rabbit Polyclonal Antibody (FITC)

SKU: orb9326

Description

NCOA3 Rabbit Polyclonal Antibody (FITC)

Images & Validation

• Tested Applications: ICC, IF
• Dilution range: IF=1:100-500, ICC/IF=1:50-200
• Reactivity: Human, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Mouse, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human SRC3 (701-800/1424aa)
• Target: NCOA3
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

NCOA3_HUMAN; Nuclear receptor coactivator 3; EC:2.3.1.48; NCoA-3; ACTR; Amplified in breast cancer 1 protein(AIB-1); CBP-interacting protein(pCIP); Class E basic helix-loop-helix protein 42(bHLHe42); Receptor-associated coactivator 3(RAC-3); Steroid receptor coactivator protein 3(SRC-3); Thyroid hormone receptor activator molecule 1(TRAM-1); AIB1; RAC3; SRC3; pCIP; AIB-1; CTG26; SRC-3; CAGH16; KAT13B; TNRC14; TNRC16; TRAM-1; bHLHe42;

Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-SRC3 antibody, dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Quick Database Links

Gene Symbol

NCOA3