Thrombomodulin Antibody [THBD/1591]

• Catalog Number: orb1252198
• Category: Antibodies
• Description: Thrombomodulin Antibody [THBD/1591]
• Target: THBD
• Clonality: Monoclonal
• Isotype: IgG2b, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 0.2 mg/mL
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Purification: Protein G affinity chromatography
• Immunogen: Human recombinant partial protein corresponding to amino acids 69-194 was used as the immunogen for this Thrombomodulin antibody.
• UniProt ID: P07204
• Tested applications: ELISA, FC, IF, IHC-P, WB
• Application notes: Flow Cytometry: 0.5-1ug/10^6 cells in 0.1mlIF: 0.5-1 ug/mlIHC (FFPE): 1-2 ug/ml for 30 min at RTELISA: 2-4 ug/ml; order the BSA-free formulation for coatingWB: 0.5-1 ug/mlThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Thrombomodulin antibody to be titered up or down for optimal performance.
• Antibody Type: Primary Antibody
• Clone Number: THBD/1591
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only

IHC testing of FFPE human cervical carcinoma with Thrombomodulin antibody (clone THBD/1591). HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human bladder carcinoma with Thrombomodulin antibody (clone THBD/1591). HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human placenta with Thrombomodulin antibody (clone THBD/1591). HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.

Western blot testing of 1) recombinant partial protein and 2) human ThP1 lysate with Thrombomodulin antibody (clone THBD/1591). Expected molecular weight ~60/100 kDa (unmodified/glycosylated).

SDS-PAGE Analysis of Purified, BSA-Free Thrombomodulin Antibody (clone THBD/1591). Confirmation of Integrity and Purity of the Antibody.