THBD Antibody

SKU: orb1252198

This product can be custom made

Description

THBD Antibody

Research Area

Cardiovascular Research

Images & Validation

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Item 1 of 5

Tested Applications	ELISA, FC, IF, IHC-P, WB
Reactivity	Human
Application Notes	Flow Cytometry: 0.5-1ug/10^6 cells in 0.1mlIF: 0.5-1 µg/mLIHC (FFPE): 1-2 µg/mL for 30 min at RTELISA: 2-4 µg/mL; order the BSA-free formulation for coatingWB: 0.5-1 µg/mLThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Thrombomodulin antibody to be titered up or down for optimal performance. Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	IgG2b, kappa
Clone No.	THBD/1591
Immunogen	Human recombinant partial protein corresponding to amino acids 69-194 was used as the immunogen for this Thrombomodulin antibody.
Target	THBD
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Concentration	0.2 mg/mL
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

IHC testing of FFPE human cervical carcinoma with Thrombomodulin antibody (clone THBD/1591). HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human bladder carcinoma with Thrombomodulin antibody (clone THBD/1591). HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human placenta with Thrombomodulin antibody (clone THBD/1591). HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.

Western blot testing of 1) recombinant partial protein and 2) human ThP1 lysate with Thrombomodulin antibody (clone THBD/1591). Expected molecular weight ~60/100 kDa (unmodified/glycosylated).

SDS-PAGE Analysis of Purified, BSA-Free Thrombomodulin Antibody (clone THBD/1591). Confirmation of Integrity and Purity of the Antibody.