CD141 antibody

SKU: orb2276264

Description

CD141 antibody validated for immunohistochemistry on 76 different Normal Tissues

Research Area

Cardiovascular Research; Immunology & Inflammation

Images & Validation

• Tested Applications: IHC
• Dilution Range: 1:100-1:200
• Reactivity: Human
• Application Notes:
  Positive Control: Lung: A strong staining should be seen in endothelial cells, and at least a weak to moderate membranous CD141 staining should be seen in alveolar macrophages. Negative Control: Colon: Kidney: Epithelial cells should not stain for CD141. Cellular Localization: Cell Surface and Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

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Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone No.: MSVA-141M
• Immunogen: Recombinant fragment (around aa 69-194) of human Thrombomodulin (CD141) protein (exact sequence is proprietary)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AHUS6; BDCA3; CD141; Fetomodulin; Thbd; THPH12; THRM; Thrombomodulin

Quick Database Links

UniProt

P07204