TGFBI Antibody

SKU: orb1249362

Description

TGFBI Antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 70kDa band observed in Human Kidney lysates (calculated MW of 74.7kDa according to Human NP_000349.1). Recommended concentration: 0.2-0.5µg/mL.Immunohistochemistry:In paraffin embedded Kidney shows staining of intercalated cells of a collecting tubule. Recommended concentration: 4-6µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-QLYTDRTEKLRPE
• Target: TGFBI
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TGFBI, transforming growth factor, beta-induced, 68kDa, BIGH3, CDB1, CDG2, CDGG1, CSD, CSD1, CSD2, CSD3, EBMD, LCD1, RGD-containing collagen-associated protein, kerato-epithelin

orb1249362 (0.3 ug/ml) staining of Human Kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1249362 (3.8 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

TGFBI

UniProt

Q15582

RefSeq (Protein):NP_000349.1