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Item 1 of 5
TGFBI Recombinant Rabbit Monoclonal Antibody
Catalog Number: orb1499365
| Catalog Number | orb1499365 |
|---|---|
| Category | Antibodies |
| Description | TGFBI Recombinant Rabbit Monoclonal Antibody |
| Species/Host | Rabbit |
| Clonality | Recombinant |
| Tested applications | IF, IHC-Fr, IHC-P, WB |
| Predicted Reactivity | Rat |
| Reactivity | Human, Mouse |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human TGFBI |
| Antibody Type | Recombinant Antibody |
| Concentration | 1mg/ml |
| Dilution range | WB=1:500-1000, IHC-P=1:100-500, IHC-F=1:400-800, IF=1:100-500 |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| MW | 72 kDa |
| Target | TGFBI |
| UniProt ID | Q15582 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Alternative names | AI181842; AI747162; beta ig; beta ig h3; beta ig-h Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-TGFBI antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (orb1499365, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-TGFBI antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (orb1499365, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-TGFBI antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (orb1499365, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-TGFBI antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (orb1499365, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of TGFBI on different lysates with Rabbit anti-TGFBI antibody (orb1499365) at 1/500 dilution. Lane 1: Mouse eyeball tissue lysate, Lane 2: Mouse colon tissue lysate, Lysates/proteins at 20 µg/Lane. Predicted band size: 75 kDa, Observed band size: 68 kDa, Exposure time: 2 minutes, 10% SDS-PAGE gel.
