TGF alpha Antibody

SKU: orb2637959

Description

TGF alpha is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. Staining with the antibody is completely blocked by the peptide used for raising this antibody.

Images & Validation

• Tested Applications: FACS, IF, IHC-P
• Dilution Range: Flow cytometry: 0.5-1ug/10^6 cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgM, kappa
• Clone No.: 1E8-G6
• Immunogen: A 10-amino acid synthetic peptide (aa 34-43) from human TGF alpha was used as the immunogen for this antibody.
• Purification: PEG precipitation
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Formalin-fixed, paraffin-embedded human testicular carcinoma stained with TGF-alpha antibody (1E8-G6)