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GDNFRA Rabbit Polyclonal Antibody

Catalog Number: orb10711

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb10711
CategoryAntibodies
DescriptionGDNFRA Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IHC-Fr, IHC-P, WB
Predicted ReactivityBovine, Canine, Equine, Porcine
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human GFR Alpha-1 (351-429/465aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Form/AppearanceLiquid
ConjugationUnconjugated
MW44 kDa
TargetGFRA1
UniProt IDP56159
RRIDAB_10762602
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesGDNF Receptor alpha 1; GFRa-1; GFR alpha; GDNFR al
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NoteFor research use only
Expiration Date12 months from date of receipt.
GDNFRA Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GDNFRA) Polyclonal Antibody, Unconjugated (orb10711) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

GDNFRA Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Human duodenum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GDNFRA) Polyclonal Antibody, Unconjugated (orb10711) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

GDNFRA Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GDNFRA) Polyclonal Antibody, Unconjugated (orb10711) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

GDNFRA Rabbit Polyclonal Antibody

Sample: Lane 1: Human HeLa cell lysates, Lane 2: Human HepG2 cell lysates, Lane 3: Human MOLT4 cell lysates, Lane 4: Human A549 cell lysates, Primary: Anti-GDNFRA (orb10711) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 44 kDa, Observed band size: 47 kDa.

GDNFRA Rabbit Polyclonal Antibody

Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Mouse Testis tissue lysates, Lane 3: Rat Cerebrum tissue lysates, Primary: Anti-GDNFRA (orb10711) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 44 kDa, Observed band size: 47 kDa.

GDNFRA Rabbit Polyclonal Antibody

Tissue/Cell: Human colon cancer, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-GDNFRA Polyclonal Antibody, Unconjugated (orb10711) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

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    IF,  IH,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 50 μl, 200 μl
  • GDNFRA Rabbit Polyclonal Antibody [orb612192]

    WB

    Bovine, Canine, Gallus, Porcine, Rabbit

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 200 μl, 50 μl
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    ELISA,  IHC-P,  WB

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    Polyclonal

    Unconjugated

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  • GFRA1 Antibody (C-term) [orb1425507]

    WB

    Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 50 μl
  • GDNFRA Rabbit Polyclonal Antibody (HRP) [orb479316]

    IHC-Fr,  IHC-P,  WB

    Bovine, Canine, Equine, Porcine

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    HRP

    100 μl