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Catalog Number | orb1240106 |
---|---|
Category | Antibodies |
Description | TFF3 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, ICC, IF, IHC-P, WB |
Predicted Reactivity | Bovine, Porcine |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | TFF3 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human TFF3.The immunogen is located within the last 50 amino acids of TFF3. |
Antibody Type | Primary Antibody |
Concentration | 1 mg/mL |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Predicted: 10 kDa Observed: 11 kDa |
Target | TFF3 |
UniProt ID | Q07654 |
NCBI | NP_003217 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | TFF3 Antibody is supplied in PBS containing 0.02% sodium azide. |
Alternative names | TFF3 Antibody: ITF, P1B, TFI, ITF, Trefoil factor Read more... |
Note | For research use only |
Application notes | TFF3 antibody can be used for detection of TFF3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL and immunocytochemistry at starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of TFF3 in human colon tissue lysate with TFF3 antibody at 1 µg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of TFF3 in human small intestine tissue with TFF3 antibody at 2.5 µg/mL.
Immunofluorescence of TFF3 in Human Small Intestine tissue with TFF3 antibody at 20 µg/mL.
Immunocytochemistry of TFF3 in HeLa cells with TFF3 antibody at 2 µg/ml.
Immunofluorescence of TFF3 in human small intestine tissue with TFF3 antibody at 20 µg/ml. Red: TFF3 Antibody (orb1240106) Blue: DAPI staining.
Immunohistochemistry of TFF3 in human small intestine tissue with TFF3 antibody at 2 µg/ml.
IF, IHC-Fr, IHC-P | |
Bovine | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC, WB | |
Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |