TFF3 Antibody

• Catalog Number: orb1240106
• Category: Antibodies
• Description: TFF3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC-P, WB
• Predicted Reactivity: Bovine, Porcine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: TFF3 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human TFF3.The immunogen is located within the last 50 amino acids of TFF3.
• Concentration: 1 mg/mL
• Dilution range: TFF3 antibody can be used for detection of TFF3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL and immunocytochemistry at starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 10 kDa Observed: 11 kDa
• Target: TFF3
• UniProt ID: Q07654
• NCBI: NP_003217
• Storage: TFF3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: TFF3 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: TFF3 Antibody: ITF, P1B, TFI, ITF, Trefoil factor
• Note: For research use only
• Application notes: TFF3 antibody can be used for detection of TFF3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL and immunocytochemistry at starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of TFF3 in human colon tissue lysate with TFF3 antibody at 1 µg/mL in (A) the absence and (B) the presence of blocking peptide.

Immunohistochemistry of TFF3 in human small intestine tissue with TFF3 antibody at 2.5 µg/mL.

Immunofluorescence of TFF3 in Human Small Intestine tissue with TFF3 antibody at 20 µg/mL.

Immunocytochemistry of TFF3 in HeLa cells with TFF3 antibody at 2 µg/ml.

Immunofluorescence of TFF3 in human small intestine tissue with TFF3 antibody at 20 µg/ml. Red: TFF3 Antibody (orb1240106) Blue: DAPI staining.

Immunohistochemistry of TFF3 in human small intestine tissue with TFF3 antibody at 2 µg/ml.