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TFF3 Rabbit Polyclonal Antibody

Catalog Number: orb11465

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb11465
CategoryAntibodies
DescriptionTFF3 Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IHC-Fr, IHC-P
Predicted ReactivityBovine
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human TFF3 (51-80/94aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeIHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Form/AppearanceLiquid
ConjugationUnconjugated
MW6.5 kDa
TargetTFF3
UniProt IDQ07654
RRIDAB_10751117
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesTrefoil Factor 3; Trefoil factor3; hP1.B; Intestin
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NoteFor research use only
Expiration Date12 months from date of receipt.
TFF3 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (TFF3) Polyclonal Antibody, Unconjugated (orb11465) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

TFF3 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Mouse small intestine), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (TFF3) Polyclonal Antibody, Unconjugated (orb11465) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

TFF3 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Mouse small intestine), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (TFF3) Polyclonal Antibody, Unconjugated (orb11465) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

TFF3 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (TFF3) Polyclonal Antibody, Unconjugated (orb11465) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

TFF3 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat small intestine), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (TFF3) Polyclonal Antibody, Unconjugated (orb11465) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

TFF3 Rabbit Polyclonal Antibody

Tissue/Cell: rat colon tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-TFF3 Polyclonal Antibody, Unconjugated (orb11465) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

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    IF

    Bovine

    Human, Mouse, Rat

    Rabbit

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    100 μl
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    ELISA,  IHC,  WB

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    IHC-Fr,  IHC-P

    Bovine

    Human, Mouse, Rat

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    Polyclonal

    HRP

    100 μl
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    Bovine

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    PE/Cy5

    100 μl
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    IF

    Bovine

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    PE/Cy5.5

    100 μl