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TFB2M Antibody

SKU: orb1249265

Description

TFB2M Antibody

Research Area

Epigenetics & Chromatin

Images & Validation

Tested ApplicationsELISA, IHC, WB
ReactivityHuman
Application Notes
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 45kDa band observed in Human Lung lysates (calculated MW of 45.3kDa according to NP_071761.1). Recommended concentration: 0.1-0.3µg/mL. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Colon. Recommended concentration: 5µg/mL.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: KHCFGRRSATVIDH
TargetTFB2M
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

TFB2M, transcription factor B2, mitochondrial, FLJ22661, FLJ23182, Hkp1, mtTFB2, HCV NS5A-transactivated protein 5, S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase 2, dimethyladenosine transferase 2, mitochondrial, h-mtTFB, h-mtTFB2, hTFB2M, hepatitis C virus NS5A-transactivated protein 5, mitochondrial 12S rRNA dimethylase 2, mitochondrial transcription factor B2

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TFB2M Antibody

orb1249265 (0.1 ug/ml) staining of Human Lung lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

TFB2M Antibody

orb1249265 (5 ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

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Datasheet
Product Information
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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

TFB2M Antibody (orb1249265)

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Available Sizes

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0.1 mg
$ 920.00
DispatchUsually dispatched within 5-10 working days
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